Test-tube embryos - mouse and human development in vitro to blastocyst stage and beyond

In vitro versus In vivo: Development-, Apoptosis-, and Implantation-Related Gene Expression in Mouse Blastocyst

Background: While mammalian embryos can adapt to their environments, their sensitivity overshadows their adaptability in suboptimal in vitro conditions. Therefore, the environment in which the gametes are fertilized or to which the embryo is exposed can greatly affect the quality of the embryo and consequently its implantation potential. Objectives:</stro...

L-carnitine decreases DNA damage and improves the in vitro blastocyst development rate in mouse embryos.

OBJECTIVE To optimize the L-carnitine (LC) concentration as a supplement in embryo culture medium and to investigate the effect of LC on developing embryos. DESIGN Experimental study. SETTING Reproductive research center at a tertiary hospital. INTERVENTION(S) To optimize the LC concentration, 420 mouse embryos were divided into seven groups and incubated with different LC concentrations ...

P-30: Developmental Capacity and Blastocyst Formation of Thawed Tetrahedral Versus Non-Tetrahedral 4-cell Stage Mouse Embryos After Vitrification

Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...

Improvement in Blastocyst Development Rate in Mouse Embryos after in Vitro Supplementation with L Carnitine (lc)

Glucose metabolism of human morula and blastocyst-stage embryos and its relationship to viability after transfer.

The pregnancy rate and implantation rate following blastocyst transfer in the human have been reported to be high; however, it has remained necessary to transfer 2-3 blastocysts to achieve these rates. Morphological criteria are currently used to select blastocysts for transfer and have some limited correlation with ongoing viability. Glucose metabolism of 189 human morula to blastocyst stage e...